【摘要】 目的 探讨将人骨髓间充质干细胞(Human bone mesenchymal stem cells ,HBMCs)诱导为内皮细胞(endothelial cells,ECs)的可行性。方法 将HBMCs分离、培养后,加入诱导因子定向诱导为ECs,并对细胞进行鉴定和细胞活性检测。结果 经鉴定,HBMCs在特定环境下分化为了ECs,而且后者与人正常血管ECs的细胞活性无显著差异性。结论 以HBMCs诱导的ECs作为种子细胞,具有可行性。

    【关键词】 人骨髓间充质干细胞;内皮细胞;种子细胞

    Experimental study of incubating HBMCs into ECs for oriented differentiation

    【Abstract】 Objective To investigate whether the method on incubating HBMCs into ECs is feasible.MethodsHBMCs were separated from human bone marrow and cultured.Then they were incubated into ECs in a special condition.The cultured cells were identified by morphological traits,the immunohistochemical method and surface markers.Results It was proved that the HBMCs was incubated into Ecs in a special condition.Conclusion the Ecs derived from HBMCs can be the ideal seeding cells to fabricate tissue engineering homologous valved conduits.

    【Key words】 HBMCs; ECs;Seeding cells

    1987年,在美国科学基金会年会上组织工程的概念被正式提出,即应用工程科学和生物科学的原理和方法,认识哺乳动物正常和病理组织器官的结构与功能关系,开发具有生物活性的人工替代物,以恢复维持和改善组织器官的功能 ......