新疆桃仁HPLC—UV指纹图谱研究及在地区分类中的应用(5)
YUN Qi， LIU Qing-wang， MA Xiao-hua， GAO Xiao-li， ZHOU Jian-ping
(1. Department of Pharmaceutics， China Pharmaceutical University， Nanjing 210009， China；
2. College of Traditional Medicine， China Pharmaceutical University， Nanjing 210009， China；
3. Xinjiang Uygur Autonomous Region Food and Drug Administration， Urumqi 830002， China；
4. College of Pharmacy， Xinjiang Medical University， Urumqi 830054， China)
[Abstract] Persicae Semen (PS)，a traditional Chinese medicine，has been widely used for the syndrome of blood stasis in China since the Eastern Han Dynasty. In the present study， we developed an HPLC-UV fingerprint analysis method for the quality control of PS. The HPLC fingerprint was performed on Shimadzu Inertsil C18 column (4.6 mm×250 mm， 5 μm) at 35 ℃. The mobile phases were composed of acetonitrile and water using a gradient elution. The flow rate was 1.0 mL·min-1，and the detection wavelength was set at 210 nm. The fingerprint method was validated according to the Guidelines for Traditional Chinese Medicine Injection Fingerprint，and applied to determine 41 batches representative herbs collected from Xinjiang of China. The chromatographic peaks were characterized by UPLC-Q-TOF-MS， and nine of them were identified by comparison with the literature and/or reference standards. In order to classify and assess the samples，hierarchical clustering analysis and partial least square discriminant analysis were performed based on the common chromatographic peaks，and the samples were geographically classified into two classes，with six chemical compounds as classification markers which were significantly different between the two classes (P<0.05).
[Key words] Persicae Semen； fingerprint； HPLC； MS； Uygur medicine
doi：10.4268/cjcmm20140521
[责任编辑 孔晶晶], 百拇医药(云琦 刘青旺 马小华 高晓黎 周建平)
(1. Department of Pharmaceutics， China Pharmaceutical University， Nanjing 210009， China；
2. College of Traditional Medicine， China Pharmaceutical University， Nanjing 210009， China；
3. Xinjiang Uygur Autonomous Region Food and Drug Administration， Urumqi 830002， China；
4. College of Pharmacy， Xinjiang Medical University， Urumqi 830054， China)
[Abstract] Persicae Semen (PS)，a traditional Chinese medicine，has been widely used for the syndrome of blood stasis in China since the Eastern Han Dynasty. In the present study， we developed an HPLC-UV fingerprint analysis method for the quality control of PS. The HPLC fingerprint was performed on Shimadzu Inertsil C18 column (4.6 mm×250 mm， 5 μm) at 35 ℃. The mobile phases were composed of acetonitrile and water using a gradient elution. The flow rate was 1.0 mL·min-1，and the detection wavelength was set at 210 nm. The fingerprint method was validated according to the Guidelines for Traditional Chinese Medicine Injection Fingerprint，and applied to determine 41 batches representative herbs collected from Xinjiang of China. The chromatographic peaks were characterized by UPLC-Q-TOF-MS， and nine of them were identified by comparison with the literature and/or reference standards. In order to classify and assess the samples，hierarchical clustering analysis and partial least square discriminant analysis were performed based on the common chromatographic peaks，and the samples were geographically classified into two classes，with six chemical compounds as classification markers which were significantly different between the two classes (P<0.05).
[Key words] Persicae Semen； fingerprint； HPLC； MS； Uygur medicine
doi：10.4268/cjcmm20140521
[责任编辑 孔晶晶], 百拇医药(云琦 刘青旺 马小华 高晓黎 周建平)